文献类型：期刊文献

英文题名：Binding of Curcumin with Bovine Serum Albumin in the Presence of ι-Carrageenan and Implications on the Stability and Antioxidant Activity of Curcumin

作者：Yang, Mingling[1];Wu, Yue[1];Li, Jinbing[1];Zhou, Haibo[1];Wang, Xiaoyong[1]

机构：[1]E China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China

年份：2013

卷号：61

期号：29

起止页码：7150

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20133116570152);WOS:【SCI-EXPANDED(收录号:WOS:000322432300017)】；

基金：This work is supported by the National Natural Science Foundation of China (Grant 21173081), the Natural Science Foundation of Shanghai (Grant 11ZR1408600), and the Fundamental Research Funds for the Central Universities (Grant WK1213003).

语种：英文

外文关键词：curcumin; BSA; iota-carrageenan; fluorescence; stability

摘要：This work studied the influences of formation of BSA/iota-carrageenan complexes on the binding, stability, and antioxidant activity of curcumin. In the presence of BSA and iota-carrageenan, curcumin gives higher intensities of absorption and fluorescence than free curcumin and curcumin only combined with BSA. The added iota-carrageenan is observed to promote curcumin for quenching the instrinsic fluorescence of BSA. These results are explained in terms of the formation of BSA/iota-carrageenan complexes, which help to stabilize the folded structure of BSA for providing curcumin with a more hydrophobic microenvironment. The small difference in anisotropy values of curcumin with BSA alone and of BSA/iota-carrageenan complexes suggests that iota-carrageenan acts as outer stretch conformation in BSA/iota-carrageenan complexes but does not directly disturb the hydrophobic pockets inside BSA, where curcumin is hydrophobically located. The determined values of the binding constant are higher for curcumin with BSA/iota-carrageenan complexes than with BSA alone. Moreover, BSA/iota-carrageenan complexes are found to be superior to single BSA for enhancing the stability and DPPH radical-scavenging ability of curcumin.