文献类型：期刊文献

英文题名：Deletion of poxB, pta, and ackA improves 1,3-propanediol production by Klebsiella pneumoniae

作者：Lin, Jie[1];Zhang, Yongqiang[1];Xu, Danfeng[1];Xiang, Gang[1];Jia, Zongxiao[1];Fu, Shuilin[1];Gong, Heng[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2016

卷号：100

期号：6

起止页码：2775

外文期刊名：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

收录：;EI(收录号：20155301738986);WOS:【SCI-EXPANDED(收录号:WOS:000371244300023)】；

基金：This work was supported by the National Natural Science Foundation of China under Grant No. 31271862.

语种：英文

外文关键词：1,3-Propanediol; Acetate; ackA; Klebsiella pneumonia; poxB; pta

摘要：To date, few studies have focused on reducing the toxic by-product acetate during 1,3-propanediol production by Klebsiella pneumoniae. In this study, the effects of deleting the poxB, pta, and ackA genes, which are involved in the two main acetate synthesis pathways, on cell growth and 1,3-propanediol production were investigated. Although acetate synthesis via pyruvate oxidase (PoxB, encoded by poxB) generally seems unnecessary and wasteful, PoxB was shown to play an important role in K. pneumoniae. Deletion of poxB severely inhibited cell growth, and the poxB mutant exhibited an anomalously high accumulation of acetate in aerobic cultures and failed to produce an endogenous supply of carbon dioxide (CO2) in anaerobic cultures. It is interesting that both the aerobic and anaerobic growth defects of the poxB mutant were corrected by further deleting pta and ackA, which blocked the other main acetate synthesis pathway. The poxB-pta-ackA mutant excreted less acetate and showed an excellent ability to produce 1,3-propandiol. The final 1,3-propanediol yield and concentration in a 2-L fed-batch fermentation reached 0.66 (mol/mol) and 76.8 g/L, respectively, which were 16 and 15 % greater, respectively, than those of the parent strain.