文献类型：期刊文献

英文题名：Protocol for engineering E. coli Nissle 1917 to diagnose, record, and ameliorate inflammatory bowel disease in mice

作者：Zou, Zhen-Ping[1];Fan, Yu-Hang[1];Du, Yue[1];Fang, Ting-Ting[1];Du, Wei[1];Zhou, Ying[1];Ye, Bang-Ce[1,2]

机构：[1]East China Univ Sci & Technol, Lab Biosyst & Microanal, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Zhejiang Univ Technol, Inst Engn Biol & Hlth, Coll Pharmaceut Sci, Collaborat Innovat Ctr Yangtze River Delta Reg Gre, Hangzhou 310014, Zhejiang, Peoples R China

年份：2023

卷号：4

期号：2

外文期刊名：STAR PROTOCOLS

收录：WOS:【ESCI(收录号:WOS:001053320400001)】；

基金：This work was sponsored by the National Natural Science Foundation of China (22134003) and the National Key Research and Development Program of China (2020YFA0908800) .

语种：英文

摘要：Engineered microorganisms hold potential for disease diagnosis and treatment. Here, we present a protocol to engineer E. coli Nissle 1917 strain (iROBOT) using genome insertion and plasmid construction to diagnose, record, and ameliorate inflammatory bowel disease in mice. We describe steps for constructing and administering iROBOT, diagnosing and recording colitis, preparing samples, and analyzing fluorescence and base editing ratios of iROBOT. We detail a colitis ameliorating assay using the disease activity index, colon length, tissue pathological section, and cytokine analysis.For complete details of the use and execution of this protocol, please refer to Zou et al.1