文献类型：期刊文献

英文题名：Metabolic transistor strategy for controlling electron transfer chain activity in Escherichia coli

作者：Wu, Hui[1,2];Tuli, Leepika[1];Bennett, George N.[3];San, Ka-Yiu[1,3]

机构：[1]Rice Univ, Dept Bioengn, Houston, TX 77005 USA;[2]E China Univ Sci & Technol, State Key Lab Biorecactor Engn, Shanghai 200237, Peoples R China;[3]Rice Univ, Dept Chem & Biomol Engn, Houston, TX 77005 USA

年份：2015

卷号：28

起止页码：159

外文期刊名：METABOLIC ENGINEERING

收录：;EI(收录号：20150600502302);WOS:【SCI-EXPANDED(收录号:WOS:000350862600017)】；

基金：The work was supported in part by a grant from the National Institutes of Health, United State (NIH GM090152).

语种：英文

外文关键词：Escherichia coli; Metabolic transistor; Electron transfer chain; Quinone; Lactate; Oxygen

摘要：A novel strategy to finely control a large metabolic flux by using a "metabolic transistor" approach was established. In this approach a small change in the level or availability of an essential component for the process is controlled by adding a competitive reaction that affects a precursor or an intermediate in its biosynthetic pathway. The change of the basal level of the essential component, considered as a base current in a transistor, has a lame effect on the flux through the major pathway. In this way, the fine-tuning of a large flux can be accomplished. The "metabolic transistor" strategy was applied to control electron transfer chain function by manipulation of the quinone synthesis pathway in Escherichin coli. The achievement of a theoretical yield of lactate production under aerobic conditions via this strategy upon manipulation of the biosynthetic pathway of the key participant, ubiquinone-8 (Q8), in an E. cull strain provides an in vivo, genetically tunable means to control the activity of the electron transfer chain and manipulate the production of reduced products while limiting consumption of oxygen to a defined amount. (C) 2015 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved