文献类型：期刊文献

英文题名：High-yield cultivation of Botryococcus braunii for biomass and hydrocarbons

作者：Wan, Minxi[1];Zhang, Zhen[1];Wang, Ruixuan[1];Bai, Wenmin[2];Huang, Jianke[1];Wang, Weiliang[1];Shen, Guomin[1];Yu, Anquan[3];Li, Yuanguang[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Jiaxing Zeyuan Bioprod Co Ltd, Jiaxing 314006, Peoples R China;[3]Yunnan Baoshan Zeyuan Microalgae Hlth Technol Co, Baoshan 678208, Peoples R China

年份：2019

卷号：131

外文期刊名：BIOMASS & BIOENERGY

收录：;EI(收录号：20194307578550);WOS:【SCI-EXPANDED(收录号:WOS:000501824400007)】；

基金：This research was partially supported by National Natural Science Foundation of China (NSFC; 21706071), China Postdoctoral Science Foundation (2018M632044), and Key Scientific and Technological Project of Shan Xi Province (Grant No. FT2014-01).

语种：英文

外文关键词：Botryococcus braunii; Hydrocarbon; Heterotrophy; Photoautotrophy

摘要：Botryococcus braunii is one of the most promising energy microalgae with a high content of hydrocarbons. Photoautotrophic cultivation is essential while the productivity is lowered by the long period of preparation for seeds. Therefore, a new strategy of photoautotrophic culture with heterotrophic seeds was proposed. The pH-auxostat fed-batch heterotrophic cultivation was developed, by which the biomass reached 37.02 g L-1 at 28 day in the 5 L fermenter. The effects of inoculum with heterotrophic seeds into photoautotrophic cultivation were verified. By this strategy, the final content and productivity of hydrocarbon was 35.9% and 128.3 mg L-1 d(-1) respectively in 1 L bubble column photo-bioreactors indoors, the highest ever reported. Furthermore, the heterotrophic seeds were successfully applied to photoautotrophic cultivation in 3 L flat panel photo-bioreactors and 120 L open circle ponds outdoors, in which the final content and productivity of hydrocarbon were 27.85% and 35.06 mg L-1 d(-1), indicating an efficient and feasible approach for producing biofuel from B. braunii.