文献类型：期刊文献

英文题名：Tandem Multimer Expression and Preparation of Hypoglycemic Peptide MC6 from Momordica charantia in Escherichia coli

作者：Wang, Fu-Jun[1,2];Song, Huai-Lei[1];Wang, Xiao-Meng[1,3];Zhang, Wen-Ji[1];Wang, Bi-Lian[1];Zhao, Jian[1];Hu, Zhi-Bi[1,2]

机构：[1]E China Univ Sci & Technol, Dept Appl Biol, Shanghai 200237, Peoples R China;[2]Shanghai Univ Chinese Tradit Med, Inst Chinese Mat Med, Shanghai 201203, Peoples R China;[3]Shanghai Jiao Tong Univ, Sch Pharm, Shanghai 200240, Peoples R China

年份：2012

卷号：166

期号：3

起止页码：612

外文期刊名：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000300296400008)】；

基金：This work was financially supported by National Key Project of Scientific and Technical Supporting Programs Funded by Ministry of Science & Technology of China (no. 2006BAI06A16).

语种：英文

外文关键词：Momordica charantia; Hypoglycemic peptide MC6; Multimer; Isocaudamer

摘要：Tandem repeat multimers of Momordica charantia (MC) peptide MC6 were designed and the recombinant plasmid containing 10 copies of MC6 gene was constructed to improve the expression level of MC6 in Escherichia coli. Under the selected conditions of cultivation and induction, the expression level of recombinant TrxA-MC6(10) protein was above 25% of total bacteria protein. This fusion protein was purified and cleaved with HCl (13%, w/v). Either the un-cleaved or cleaved recombinant proteins was analyzed pharmacological activity by alloxan-induced diabetic mice and only the cleaved products of the recombinant protein showed significant hypoglycemic effects. The study provides a convenient and economical method for the large-scale production of anti-diabetic medicines for pharmaceutical applications.