文献类型：期刊文献

英文题名：Chain flexibility and branching effects on arabinogalactan conformation in solution

作者：Huang, Hai[1];Wu, Mengqi[2];Takahashi, Rintaro[1];Zhang, Wenqing[2];Terao, Ken[1]

机构：[1]Univ Osaka, Grad Sch Sci, Dept Macromol Sci, 1-1 Machikaneyama Cho,Toyonaka, Toyonaka, Osaka 5600043, Japan;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China

年份：2025

卷号：370

外文期刊名：CARBOHYDRATE POLYMERS

收录：;EI(收录号：20253719167498);WOS:【SCI-EXPANDED(收录号:WOS:001573225600001)】；

基金：The authors are grateful to Dr. Noboru Ohta (SPring-8) for the SAXS measurements. The synchrotron radiation experiments were performed at the BL40B2 in SPring-8 with the approval of the Japan Synchrotron Radiation Research Institute (JASRI) (proposal Nos. 2023A1111, 2023A1112, 2023B1124, 2023B1125, 2024A1118, 2024A1119, 2024B1152, and 2024B1153). The NMR and CHN analysis measurements were performed at Analytical Instrument Facility, Graduate School of Science, The University of Osaka. This work was partially supported by JSPS KAKENHI grant Nos. JP23H02011 and JP23K26704.

语种：英文

外文关键词：Arabinogalactan; Small-angle X-ray scattering; Radius of gyration; Hydrodynamic radius; Form factor; Intrinsic viscosity

摘要：The structural and conformational characteristics of three arabinogalactans were investigated in aqueous and aprotic organic solutions using small-angle X-ray scattering (SAXS), dynamic light scattering, and viscosity measurements. This study aimed to clarify the dimensional and hydrodynamic properties related to the branching structure of a type II arabinogalactan extracted from jasmine tea leaves by comparing it with linear arabinogalactan (LG) and highly branched larch arabinogalactan (LAG60) samples. The obtained form factor P (q) and the radius of gyration Rg for LG were analyzed in terms of the wormlike chain model with the Kuhn segment length of 2.80 nm, a measure of chain stiffness. This result indicates higher chain flexibility than that of polysaccharides whose backbone consists of 1,4-linked hexoses in a good solvent. The P(q) data of the other two arabinogalactans, LAG60 and JSP-1a, which have short branches, were well described by the hyperbranched chain model. This model considers the fractal dimensions of partial chains in good solvent systems. The model uses a fixed number of branching points that exclude the contribution of single-sugar-residue branches. In addition, the hydrodynamic properties and the second virial coefficient were consistent with the P(q) and Rg results.