文献类型：期刊文献

中文题名：延长盐单胞菌生产依克多因高密度发酵工艺优化

英文题名：High-Density Fermentation Process Optimization for Ectoine production by Halomonas elongata DSM 2581^(T)

作者：张悦[1];刘昊[1];刘玮[1];余君雄[1];肖扬[1];郇晶伟[1];颜诚旭[1];庄英萍[1];王泽建[1]

机构：[1]华东理工大学生物工程学院,上海200237

年份：2025

卷号：51

期号：1

起止页码：50

中文期刊名：华东理工大学学报(自然科学版)

外文期刊名：Journal of East China University of Science and Technology

收录：;北大核心:【北大核心2023】；

基金：国家重大专项项目(2020YFA096800)。

语种：中文

中文关键词：延长盐单胞菌;合成培养基;高细胞密度培养;依克多因;盐冲击

外文关键词：Halomonas elongata;defined medium;high cell density culture;ectoine;salt shock

摘要：建立了延长盐单胞菌(Halomonas elongata DSM 2581T)高效合成依克多因的高密度发酵工艺。通过单因素实验、Plackett-Burman实验与Central Composite Design系统考察了合成培养基营养组分对H.elongata DSM 2581T发酵代谢的影响。优化结果表明:3 g/L柠檬酸钠,3 g/L氯化铵,80 g/L氯化钠的培养基组分能够显著促进H.elongata DSM 2581T菌体的生长和产物合成。根据H.elongata DSM 2581T在5 L发酵罐中优化后的培养基条件下的生长代谢情况,建立了葡萄糖连续流加和pH反馈控制(氨水)的补料方式进行H.elongata DSM 2581T高密度发酵工艺,实施了将对数生长期发酵液中氯化钠质量浓度从80 g/L提高到130 g/L的快速盐梯度冲击策略,发酵13 h后OD600最高达到169.3,依克多因产量和合成速率分别达到20 g/L和1.54 g/(L·h)。这项研究为实现依克多因的低成本、高效率工业化生产提供了理论指导。
In this study,a high-density fermentation process of Halomonas elongate DSM 2581^(T) was established,which had the advantage of high ectoine production rate.The effects of nutrient components of defined medium on fermentation and metabolism of H.elongata DSM 2581^(T) were investigated by single factor experiment,Plackett-Burman and Central Composite Design.The results showed that sodium citrate(3 g/L),NH_(4)Cl(3 g/L)and NaCl(80 g/L)could significantly promote the growth and ectoine production of H.elongata DSM 2581^(T).Furthermore,according to the growth and metabolism of H.elongata in the defined medium in a 5 L fermentor,the feeding mode of glucose continuous flow plus and pH feedback control(ammonia water)was established to carry out the high-density fermentation process of H.elongata DSM 2581^(T).Salt shock,which increased the NaCl concentration in the fermentation broth from 80 g/L to 130 g/L in the exponential growth phase,resulted in OD_(600) reaching 169.3 after 13 h of fermentation,with ectoine production and ectoine synthesis reaching 20 g/L and 1.54 g/(L·h),respectively.This study provides theoretical guidance for the low-cost and efficient industrial ectoine production.