文献类型：期刊文献

英文题名：Super-Photostability and Super-Brightness of EC5 Dyes for Super-Resolution Microscopy in the Deep Near-Infrared Spectral Region

作者：Lu, Xicun[1]; Zhuang, Xiaoli[1]; Dong, Yan[1]; Chen, Chong[2]; Wei, Ruwei[1]; Chen, Weichao[1]; Li, Hui[2]; Luo, Xiao[3]; Qian, Xuhong[1,3]; Yang, Youjun[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of Chemical Biology, School of Pharmacy, East China University of Science and Technology, Shanghai, 200237, China; [2] Jiangsu Key Laboratory of Medical Optics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, 215163, China; [3] Shanghai Engineering Research Center of Molecular Therapeutics and New Drug Development, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai, 200241, China

年份：2024

卷号：36

期号：2

起止页码：949

外文期刊名：Chemistry of Materials

收录：EI(收录号：20240215340217)

语种：英文

外文关键词：Diffraction - Infrared devices - Optical resolving power

摘要：The confocal and super-resolution imaging has become routine for the visible spectral region, but not for the deep near-infrared (NIR) region (800-1000 nm) due to the lack of bright and ultraphotostable fluorophores. We devised a premium fluorochromic scaffold (EC5) spectrally active in this region and further optimized it for super-resolution imaging by rational and systematic molecular engineering over the push-pull headgroups. EC5j maximally absorbs/emits at 835/873 nm and offers a superior brightness of 38070 cm-1 M-1 and superior photostability. Using two home-built microscopes with deep-NIR capability, i.e., confocal and structured-illumination microscopes, we demonstrated that super-resolution 2D and 3D-microscopy is now practical with EC5j. In particular, EC5j-adsorbed polystyrene beads furnished a notable spatial resolution of 175 nm with structure-illumination microscopy (SIM) imaging, as compared to the theoretical diffraction limit of 336 nm. Proof-of-concept applications included live-cell confocal imaging of mitophagy, SIM imaging of mitochondria, and deconvolution-based confocal sectioning of cell structures and brain vasculature. The revolutionary expansion of the imaging spectral window brings the field new tools and new insights. ? 2023 American Chemical Society