文献类型：期刊文献

英文题名：A fluorescent light-up probe as an inhibitor of intracellular β-tryptase

作者：Wang, Qi[1];Shi, Xiuyin[2];Zhu, Xiaoxia[1];Ehlers, Martin[3];Wu, Junchen[4,5];Schmuck, Carsten[3]

机构：[1]Nantong Univ, Coll Publ Hlth, Nantong 226019, Peoples R China;[2]Nantong Univ, Affiliated Hosp, Med Lab Ctr, Nantong 226019, Peoples R China;[3]Univ Duisburg Essen, Inst Organ Chem, Essen, Germany;[4]E China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[5]E China Univ Sci & Technol, Inst Fine Chem, Shanghai 200237, Peoples R China

年份：2014

卷号：50

期号：46

起止页码：6120

外文期刊名：CHEMICAL COMMUNICATIONS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000336254000011)】；

基金：This work was funded by the National Basic Research 973 Program (2013CB733700) and the Fundamental Research Funds for the Central Universities (WJ1213007), the innovation program of shanghai municipal education commission (J100-2-13104), the Program of Shanghai Pujiang (K100-2-1275), the National Natural Science Foundation of China (21341001), Natural Science Foundation of Nantong University (12ZY034) and the German Science Foundation (DFG) (SFB 1093).

语种：英文

摘要：A pyrene-functionalized peptidic inhibitor 1 binds to and inhibits beta-tryptase in a non-competitive and reversible manner even in cells. Upon protein binding a fluorescence increase of the two pyrene fluorophores is observed which allows using 1 as a fluorescent light-up probe for this enzyme.