文献类型：期刊文献

英文题名：Drug-carrier/hydrogel scaffold for controlled growth of cells

作者：Wei, Lan[1];Lin, Jiaping[1];Cai, Chunhua[1];Fang, Zhengdong[2];Fu, Weiguo[2]

机构：[1]E China Univ Sci & Technol, Shanghai Key Lab Adv Polymer Mat, State Key Lab Bioreactor Engn, Sch Mat Sci & Engn, Shanghai 200237, Peoples R China;[2]Fudan Univ, Dept Vasc Surg, Shanghai 200433, Peoples R China

年份：2011

卷号：78

期号：3

起止页码：346

外文期刊名：EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000294105600006)】；

基金：This work was supported by National Natural Science Foundation of China (50925308). Supports from projects of Shanghai municipality (09XD1401400, 0952nm05100, 08DZ2230500, and B502) are also appreciated.

语种：英文

外文关键词：Scaffold; Drug delivery; Hydrogel; Micelle; Alginate

摘要：In this work, a novel functional drug-carrier/hydrogel scaffold was prepared to control the growth of cells for tissue engineering. The drug-carrier/hydrogel scaffold was constructed from a micelle/Ca-alginate microparticles (Alg-MPs)/poly(vinyl alcohol) (PVA) hydrogel composite. In such a system, paclitaxel (PTX) is encapsulated in the micelles formed by poly(L-glutamic acid)-b-poly(propylene oxide)b-poly(L-glutamic acid) (GPG), while human vascular endothelial growth factor-165 (VEGF(165)) is loaded in the Alg-MPs. The designed function of this scaffold is to encourage the fast growth of cells such as endothelial cells (ECs) in the early period to reduce the rejection and inhibit the growth of cells such as smooth muscle cells (SMCs) in late period to prevent the vascular intimal hyperplasia. The effect of VEGF(165) is to encourage the growth of ECs. while PTX is used to inhibit the growth of smooth muscle cells (SMCs). Structure characterizations show that the drug carriers are well dispersed in the PVA hydrogel. Independent release behaviors of the two drugs are observed. VEGF(165) shows a short-term release behavior, while PTX shows a long-term release behavior from the drug-carrier/hydrogel scaffolds. Further study shows a controllable cell growth behavior on this functional drug-carrier/hydrogel scaffold via the MTT assay. (C) 2011 Elsevier B.V. All rights reserved.