文献类型：期刊文献

英文题名：High-level production of bovine enterokinase light chain using fed-batches by recombinant Pichia pastoris

作者：Zhang, J.-G.[1]; Wang, X.-D.[1]; Mao, X.-Z.[1]; Wei, D.-Z.[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China

年份：2009

卷号：23

期号：2

起止页码：219

外文期刊名：Chemical and Biochemical Engineering Quarterly

收录：EI(收录号：20094912523259)

语种：英文

外文关键词：Bioreactors - Mammals - Cells - Chains - Cytology - Yeast

摘要：A recombinant Pichia pastoris containing enterokinase light chain gene from bovine was cultured in flasks firstly at different pH, methanol addition, and cell mass concentration for enterokinase production. Activity of enterokinase increased as cell mass concentration increased at more than Φ = 6% methanol added. No significant change of activity of enterokinase was observed when methanol added was less than Φ = 4 %. No activity of enterokinase lost after 120 h conservation at different pH. Secondly, high-level enterokinase production was achieved in 3.7 L bioreactor at pH 4.0, Φ = 0.6 % methanol, p = 52.5 kPa. Activity of enterokinase was not cell mass concentration dependent at Φ = 0.6 % methanol in bioreactor. Yield of enterokinase was 479.99 mg L-1 after 97.5 h induction. Maintenance coefficient and methanol consumed were calculated to analyse the enterokinase production.