文献类型：期刊文献

英文题名：Investigation of factors affecting biotransformation of phytosterols to 9-hydroxyandrost-4-ene-3,-17-dione based on the HP-β-CD-resting cells reaction system

作者：Gao, Xingqiang[1];Feng, Jianxun[1];Hua, Qiang[1];Wei, Dongzhi[1];Wang, Xuedong[1]

机构：[1]E China Univ Sci & Technol, Sch Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2014

卷号：32

期号：5-6

起止页码：343

外文期刊名：BIOCATALYSIS AND BIOTRANSFORMATION

收录：;EI(收录号：20150100401341);WOS:【SCI-EXPANDED(收录号:WOS:000346976400012)】；

基金：This paper is supported by the National Natural Science Foundation of China (General Program, No. 21276083).

语种：英文

外文关键词：Biotransformation; hydroxypropyl-beta-cyclodextrin; Mycobacterium; phytosterols; rate limitation

摘要：Based on the hydroxypropyl-beta-cyclodextrin-resting cells reaction system, high substrate concentration and improved reaction rate were achieved in a bioprocess of microbial side-chain cleavage of phytosterols to 9-OH-AD. Investigation of the kinetics of biotransformation showed that the initial reaction rate was strongly inhibited by a substrate concentration greater than 8 g/L. The oxygen transfer rate was also a critical parameter that could influence the rate of reaction. The results highlighted the importance of dissolved oxygen, particularly at high substrate concentration. Finally, analysis of metabolites showed that the main loss of process yield was due to by-product (16.3%) and nucleus degradation (8-11%) in the biotransformation. Investigation of the kinetics of metabolite formation provided fundamental knowledge on the biochemical reaction which was critical for understanding how to develop the biotransformation into an industrial application.