文献类型：期刊文献

中文题名：以组织工程技术构建活性双层人工皮肤

英文题名：Constitution of Bilayered Living Skin by Seeding Human Dermal Fibroblasts and Epidermal Keratinocytes on Collagen Sponge in vitro

作者：陈卫[1];周燕[1];华平[1];谭文松[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2003

卷号：29

期号：5

起止页码：467

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;国家哲学社会科学学术期刊数据库;EI(收录号：2003507781747);Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

基金：国家高技术研究发展计划课题资助(2001AA210641);国家重点基础研究发展规划"组织工程的基本科学问题"项目资助(G1999054309)

语种：中文

中文关键词：组织工程;胶原海绵;角质细胞;成纤维细胞;活性双层人工皮肤

外文关键词：tissue engineering; collagen sponge; keratinocyte; fibroblasts; bilayered living skin

摘要：用冷冻干燥胶原凝胶方法制备胶原海绵,经戊二醛交联处理,作为活性双层人工皮肤的支架;以幼儿包皮为细胞来源获得真皮成纤维细胞和皮肤角质细胞,体外扩增后作为种子细胞;在胶原海绵上接种成纤维细胞和角质细胞,体外构建活性双层人工皮肤。研究结果表明,人工真皮内的成纤维细胞生长良好,并且有人纤粘连蛋白类生物活性物质存在,活性双层人工皮肤表皮层有类似于天然皮肤表皮层的组织结构。
Collagen sponge using as artificial skin scaffolds was manufactured by freezedrying collagen gel and crosslinked with glutaraldehyde. Human dermal fibroblasts and human epidermal keratinocytes were derived from infant foreskin and proliferated in vitro. Then bilayered living skin was constituted on collagen sponges by seeding human dermal fibroblasts and human epidermal keratinocytes, and cultured in vitro. It was shown that fibroblasts not only adhered, grew and proliferated properly, but also produced fibronectin in artificial dermis. The epidermal histologic appearance of bilayered living skin was found to be similar to that of normal skin.