文献类型：期刊文献

英文题名：A Novel Layered Anchoring Structure Immobilized Cellulase via Covalent Binding of Cellulase on MNPs Anchored by LDHs

作者：Pei, Jicong[1];Huang, Yan[1];Yang, Yuxiang[1,2];Yuan, Hongming[3];Liu, Xiangnong[4];Ni, Chaoying[2]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[2]Univ Delaware, Dept Mat Sci & Engn, Newark, DE 19716 USA;[3]Jilin Univ, State Key Lab Inorgan Synth & Preparat Chem, Changchun 130012, Jilin, Peoples R China;[4]Yangzhou Univ, Anal Test Ctr, Yangzhou 225009, Jiangsu, Peoples R China

年份：2018

卷号：28

期号：4

起止页码：1624

外文期刊名：JOURNAL OF INORGANIC AND ORGANOMETALLIC POLYMERS AND MATERIALS

收录：;EI(收录号：20181304959869);WOS:【SCI-EXPANDED(收录号:WOS:000435603300025)】；

基金：This work was supported by the National Natural Science Foundation of China (20577010, 20971043); and the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：Layered double hydroxides; Fe3O4 magnetic fluid; Immobilized cellulase; Layered anchoring structure

摘要：In this study, Fe3O4 nanoparticles were first synthesized by co-precipitation method and then layered double hydroxides (LDHs) nanosheets were anchored onto Fe3O4 via an in-situ growth method to prepare LDHs@Fe3O4 carrier. The obtained magnets and LDHs@Fe3O4 carrier were characterized by XRD, FT-IR, N-2 adsorption, HRSEM and HRTEM respectively. Using glutaraldehyde as a coupling agent, cellulase was immobilized onto this magnetic carrier by covalent binding. The effects of various parameters on enzyme activity of LDHs@Fe3O4 immobilized cellulase, including cellulase concentration, glutaraldehyde concentration, crosslinking time, pH, cellulase concentration, immobilization time and temperature were discussed in detailed. Moreover, thermal stability and operating stability of the immobilized cellulase were studied, the loaded amount of cellulase was measured, and the degradation performance of methoxychlor (MXC) by layered anchoring structure immobilized cellulase was evaluated.