文献类型：期刊文献

中文题名：反义核酸对肿瘤细胞多药抗性的抑制——作用靶点及作用方式的研究

英文题名：Inhibition of Multidrug Resistance in Human Tumor Cell by Antisense Oligodeoxynucleotides

作者：钱江潮[1];魏东芝[1];张嗣良[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,生物化学研究所上海200237

年份：2000

卷号：26

期号：2

起止页码：157

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：EI(收录号：2000075253519);北大核心:【北大核心1996】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金!3 9680 0 3 2;国家科委医药技术创新博士项目;青年863项目

语种：中文

中文关键词：多药抗性;反义核酸;肿瘤细胞;化疗药物;抑制

外文关键词：multidrug resistance; P glycoprotein; oligodeoxynucleotide; S modified oligodeoxynucleotide; ATP binding site

摘要：为了用反义寡聚 DNA调控抗阿霉素肿瘤细胞 KB- A- 1的多药抗性 ,对反义核酸的预期作用位点、作用方式和硫代化类似物进行了研究。筛选得到两段对应于翻译起始区 ( 3)和 P-糖蛋白ATP结合位点 ( 9)的 2 0聚反义片段 ,发现反义核酸的使用次数对结果的检测有很大影响 ,采用5μmol/L的反义片段 9连续处理 4d,实现了将近 50 %抑制效果 ,而其正义和错义链在 1 0 μmol/L浓度的同样处理条件下则没有检测到抑制效果。还表明硫代化反义核酸在没有助转染剂时效果不如天然片段。
Antisense oligodeoxynucleotides (ODN) were studied to reverse the multidrug resistance of an adriamycin resistant cell line KB A 1. The antisense target, administration mode and the effect of phosphorothioate ODN were investigated. Two 20 mer ODNs(3 and 9), according to translation initiation site and ATP binding site respectively, were selected and a nearly 50% inhibition of drug resistance was obtained with sequence 9 at the concentration of 5μmol/L for 4 days. Meanwhile, the sense and missense ODNs could not exhibit inhibition at the concentration of 10μmol/L for 4 days. The data also demonstrated that phosphorothioate ODN was not as effective as the natural ODN in the condition of no lipofectin present .