文献类型：期刊文献

英文题名：Enhancement of 9α-Hydroxy-4-androstene-3,17-dione Production from Soybean Phytosterols by Deficiency of a Regulated Intramembrane Proteolysis Metalloprotease in Mycobacterium neoaurum

作者：Xiong, Liang-Bin[1];Sun, Wan-Ju[1];Liu, Yong Jun[1];Wang, Feng-Qing[1];Wei, Dong-Zhi[1]

机构：[1]East China Univ Sci & Technol, Newworld Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2017

卷号：65

期号：48

起止页码：10520

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20175004529106);WOS:【SCI-EXPANDED(收录号:WOS:000417670500016)】；

基金：This research was financially supported by the National Natural Science Foundation of China (Grant 31370080) and the National Special Fund for State Key Laboratory of Bioreactor Engineering.

语种：英文

外文关键词：Mycobacterium; 9 alpha-hydroxy-4-androstene-3,17-dione (9-OHAD); soybean phytosterol; rip1; sigD

摘要：Modification of the sterol catabolism pathway in mycobacteria may result in the accumulation of some valuable steroid pharmaceutical intermediates, such as 9 alpha-hydroxy-4-androstene-3,17-dione (9-OHAD). In previous work, sigma factor D (SigD) was identified as a negative factor of the 9-OHAD production in Mycobacterium neoaurum. Here, the deficiency of rip1 putatively coding for a regulated intramembrane proteolysis metalloprotease (Rip1), which could cleave the negative regulator of SigD (anti-SigD), enhanced the transcription of some key genes (choM1, kshA, and hsd4A) in the sterol catabolic pathway. Furthermore, the deletion of rip1 increased the consumption of phytosterols by 37.8% after 96 h of growth in M. neoaurum. The production of 9-OHAD in the engineered M. neoaurum Delta kstD1 Delta kstD2 Delta kstD3 Delta ripl (Mn Delta k123 Delta rip1) strain was ultimately increased by 27.3% compared to that in its parental strain M. neoaurum Delta kstD1 Delta kstD2 Delta kstD3 (Mn Delta k123). This study further confirms the important role of SigD-related factors in the catabolism of sterols.