文献类型：期刊文献

英文题名：A novel method based on ligase detection reaction for low abundant YIDD mutants detection in hepatitis B virus

作者：Xiao, ZX; Xiao, JX; Jiang, YX; Zhang, SY; Yu, MH; Zhao, JL; Wei, DZ; Cao, HM

机构：[1]Chinese Acad Sci, Shanghai Inst Microsyst & Informat Technol, Shanghai 200050, Peoples R China;[2]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, New World Inst Biotechnol, Shanghai 200237, Peoples R China;[3]Wuxi Infect Dis Hosp, Wuxi 214005, Peoples R China

年份：2006

卷号：34

期号：3

起止页码：150

外文期刊名：HEPATOLOGY RESEARCH

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000236532400004)】；

语种：英文

外文关键词：LDR/PCR; HBV; YMDD; YIDD

摘要：A novel method based on ligase detection reaction (LDR) coupled with polymerase chain reaction (PCR) was used in this study to detect low abundant YIDD mutants in hepatitis B virus (HBV), which was able to detect 10(2) mutants from 10(8) wild type copies in plasmids and 10(3) from 10(7) in clinical serum samples. Direct sequencing and microarray hybridization were selected for sensitivity comparison together with this assay. By direct sequencing, 6 of 50 clinical specimens infected with HBV were found to be YIDD and the others to be YMDD, while by microarray hybridization, 28 specimens were detected to contain both YMDD and YIDD, and the others contained YMDD only, and with this assay, 31 specimens containing both YMDD and YIDD were detected with the others containing YMDD only, which indicated that the assay was relatively more sensitive. (c) 2006 Elsevier Ireland Ltd. All rights reserved.