文献类型：期刊文献

英文题名：Anamperometric superoxide anion radicalbiosensor based on SOD/PtPd-PDARGO modified electrode

作者：Tang, Jie[1];Zhu, Xiang[1];Niu, Xiangheng[1];Liu, Tingting[1];Zhao, Hongli[1];Lan, Minbo[1,2]

机构：[1]E China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2015

卷号：137

起止页码：18

外文期刊名：TALANTA

收录：;EI(收录号：20150600503558);WOS:【SCI-EXPANDED(收录号:WOS:000351656200003)】；

基金：This research was financially supported by National Natural Science Foundation of China (NSFC, no. 21305044), Science and Technology Commission of Shanghai Municipality (STCSM, no. 13510710900), and the Fundamental Research Funds for the Central Universities (No. 222201314026).

语种：英文

外文关键词：Superoxide anion radical; PtPd NPs; PDARGO; Superoxide dismutase

摘要：In the present work, a high-performance enzyme-based electrochemical sensor for the detection of superoxide anion radical (O-2(center dot-)) is reported. Firstly, we employed a facile approach to synthesize PtPd nanoparticles (PtPd NPs) on chemically reduced graphene oxide (RGO) coated with polydopamine (PDA). The prepared PtPd-PDARGO composite was well characterized by transmission electron microscopy, scanning electron microscopy, Fourier transform infrared spectra, X-ray diffraction, X-ray photoelectron spectroscopy and electrochemical methods. Then the assembled composite was used as a desired electrochemcial interface for superoxide dismutase (SOD) immobilization. Owing to the PDA layer as well as the synergistic effect of PtPd NPs, the fabricated SOD/PtPd-PDARGO sensor exhibited an outstanding sensitivity of 909.7 mu A mM(-1) cm(-2) upon O-2(center dot-) in a linear range from 0.016 mM to 0.24 mM (R-2=0.992), with a low detection limit of 2 mu M (S/N=3) and excellent selectivity, good reproducibility as well as favorable long-term stability. (C) 2015 Elsevier B.V. All rights reserved.