文献类型：期刊文献

英文题名：Comparative omics directed gene discovery and rewiring for normal temperature-adaptive red pigment synthesis by polar psychrotrophic fungus Geomyces sp. WNF-15A

作者：Long, Haoyu[1];Zhou, Jiawei[1];Ren, Yanna[1];Lu, Jian[1];Wang, Nengfei[2];Liu, Haifeng[3];Zhou, Xiangshan[4];Cai, Menghao[1,5]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Linyi Univ, Sch Chem & Chem Engn, Linyi 276005, Peoples R China;[3]China Resources Angde Biotech Pharm Co Ltd, 78 E Jiao St, Liaocheng 252201, Peoples R China;[4]China Resources Biopharmaceut Co Ltd, 1301-84 Sightseeing Rd, Shenzhen, Peoples R China;[5]Shanghai Collaborat Innovat Ctr Biomfg, Shanghai 200237, Peoples R China

年份：2024

卷号：9

期号：4

起止页码：842

外文期刊名：SYNTHETIC AND SYSTEMS BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001273420100001)】；

基金：This study was supported by the National Natural Science Foundation of China (42176238) and the 111 Project of China (B18022). We thank Shanghai Personalbio Technology Co., Ltd. for the support of genome sequencing, and Shanghai Sangon Biotech Co., Ltd. for the support of metabolome sequencing.

语种：英文

外文关键词：Polar fungi; Red pigment; Cold-adaptive; Comparative omics; Food additives

摘要：The Antarctic fungus Geomyces sp. WNF-15A can produce high-quality red pigments (AGRP) with good prospects for the use in food and cosmetic area. However, efficient AGRP synthesis relies on low-temperature and thus limits its industrial development. Here genome sequencing and comparative analysis were performed on the wild-type versus to four mutants derived from natural mutagenesis and transposon insertion mutation. Eleven mutated genes were identified from 2309 SNPs and 256 Indels. A CRISPR-Cas9 gene-editing system was established for functional analysis of these genes. Deficiency of scaffold1.t692 and scaffold2.t704 with unknown functions highly improved AGRP synthesis at all tested temperatures. Of note, the two mutants produced comparable levels of AGRP at 20 degrees C to the wild-type at 14 degrees C. They also broke the normal-temperature limitation and effectively synthesized AGRP at 25 degrees C. Comparative metabolomic analysis revealed that deficiency of scaffold1.t692 improved AGRP synthesis by regulation of global metabolic pathways especially downregulation of the competitive pathways. Knockout of key genes responsible for the differential metabolites confirmed the metabolomic results. This study shows new clues for cold-adaptive regulatory mechanism of polar fungi. It also provides references for exploitation and utilization of psychrotrophic fungal resources.