文献类型：期刊文献

英文题名：Remodeling metabolism of Corynebacterium glutamicum for high-level dencichine production

作者：Huang, Dan[1];Wang, Xin[1];Liu, Wei-Bing[1];Ye, Bang-Ce[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Lab Biosyst & Microanal, Shanghai 200237, Peoples R China

年份：2023

卷号：388

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20234014819904);WOS:【SCI-EXPANDED(收录号:WOS:001086133500001)】；

基金：This work was supported by National Key Research and Develop- ment Program of China (2021YFC2101500) . Grateful thanks to Profes- sor Xin-Xiao Sun for his technical support.

语种：英文

外文关键词：Corynebacterium glutamicum; L-2, 3-diaminopropionate; Oxalyl-coenzyme A; Dencichine; Metabolic engineering

摘要：Dencichine, a sought-after compound in the medical industry, requires a more efficient and sustainable production method than the current plant extraction process. This study successfully remodeled the metabolic pathway of Corynebacterium glutamicum to produce dencichine from the precursors of L-2,3-diaminopropionate (L-DAP) and oxalyl-coenzyme A. Firstly, a synthetic pathway for L-DAP was established by introducing exogenous enzymes ZmaU/ZmaV. This resulted in a production of 628 mg/L by overexpressing key genes and reducing the endogenous competitive pathway. Secondly, an oxalyl-CoA synthetic pathway was created through the enzymatic conversion of glyoxylate by introducing heterologous enzymes. Finally, with the integration of the exogenous enzyme BAHD, de novo synthesis of dencichine in C. glutamicum was achieved, and production reached 31.75 mg/L within 48-hour fermentation. This achievement represents the first successful biosynthesis of dencichine in C. glutamicum, offering a promising approach for natural product through microbial fermentation.