文献类型：期刊文献

英文题名：An Integrated Control Strategy for the Fermentation of the Marine-Derived Fungus Aspergillus glaucus for the Production of Anti-cancer Polyketide

作者：Cai, Menghao[1]; Zhou, Xiangshan[1]; Lu, Jian[1]; Fan, Weimin[1]; Zhou, Jiushun[1]; Niu, Chuanpeng[1]; Kang, Li[1]; Sun, Xueqian[1]; Zhang, Yuanxing[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China

年份：2012

卷号：14

期号：6

起止页码：665

外文期刊名：Marine Biotechnology

收录：EI(收录号：20124715693147)

语种：英文

外文关键词：Biochemistry - Bioreactors - Shear stress - Aspergillus - Bioconversion - Metabolites - Sodium hydroxide - Diseases - Dissolved oxygen - Ketones

摘要：An integrated control strategy of pH, shear stress, and dissolved oxygen tension (DOT) for fermentation scale-up of the marine-derived fungus Aspergillus glaucus HB 1-19 for the production of the anti-cancer compound aspergiolide A was studied. Keeping initial pH of 6. 5 and shifting pH from 6. 0 to 7. 0 intermittently during the production phase greatly facilitated biosynthesis of aspergiolide A in shake flask cultures. Thus, a pH-shift strategy was proposed that shifting pH to 7. 0 once it went lower than 6. 0 by pulsed feeding NaOH solution during the production phase in bioreactor fermentation of A. glaucus HB 1-19. As a result, aspergiolide A production in a 30-L bioreactor was increased to 37. 6 mg/L, which was 48. 6% higher than that in 5-L bioreactor without pH shift. Fermentation scale-up was then performed in a 500-L bioreactor on the basis of an integrated criterion of near-same impeller tip velocity of early phase, DOT levels, and pH shift. The production of aspergiolide A was successfully obtained as 32. 0 mg/L, which was well maintained during the process scale-up. This work offers useful information for process development of large-scale production of marine microbial metabolites. ? 2012 Springer Science+Business Media, LLC.