文献类型：期刊文献

英文题名：A light-switchable bidirectional expression module allowing simultaneous regulation of multiple genes

作者：Chen, Xianjun[1,2,3];Li, Ting[1,2,3];Wang, Xue[1];Yang, Yi[1,2,3]

机构：[1]E China Univ Sci & Technol, Synthet Biol & Biotechnol Lab, State Key Lab Bioreactor Engn, Shanghai Collaborat Innovat Ctr Biomfg Technol, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, CAS Ctr Excellence Brain Sci, Optogenet & Mol Imaging Interdisciplinary Res Ctr, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China

年份：2015

卷号：465

期号：4

起止页码：769

外文期刊名：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000361782500019)】；

基金：This work was supported by the NSFC (31225008, 91313301, 31170815 and 31470833), Shanghai Science and Technology Commission (12JC1402900, 11DZ2260600, 15YF1402600), State Key Laboratory of Bioreactor Engineering, the 111 Project (B07023), and the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：Bidirectional; Gene expression; Light; Simultaneous regulation

摘要：Several light-regulated genetic circuits have been applied to spatiotemporally control transgene expression in mammalian cells. However, simultaneous regulation of multiple genes using one genetic device by light has not yet been reported. In this study, we engineered a bidirectional expression module based on LightOn system. Our data showed that both reporter genes could be regulated at defined and quantitative levels. Simultaneous regulation of four genes was further achieved in cultured cells and mice. Additionally, we successfully utilized the bidirectional expression module to monitor the expression of a suicide gene, showing potential for photodynamic gene therapy. Collectively, we provide a robust and useful tool to simultaneously control multiple genes expression by light, which will be widely used in biomedical research and biotechnology. (C) 2015 Elsevier Inc. All rights reserved.