文献类型：期刊文献

英文题名：Selective separation of flavonoid glycosides in Dalbergia odorifera by matrix solid-phase dispersion using titania

作者：Xu, Lingyan[1]; Shi, Hui[1]; Liang, Tu[1]; Feng, Jiatao[2]; Jin, Yu[1,3]; Ke, Yanxiong[1]; Liang, Xinmiao[1]

机构：[1] Engineering Research Center of Pharmaceutical Process Chemistry, Ministry of Education, East China University of Science and Technology, Shanghai, China; [2] Dalian Institute of Chemical Physics, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, Dalian, China; [3] School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China

年份：2011

卷号：34

期号：11

起止页码：1347

外文期刊名：Journal of Separation Science

收录：EI(收录号：20112314046385)

语种：英文

外文关键词：Separation - Acetonitrile - Dispersions - Titanium dioxide - Sugars - Flavonoids

摘要：Dalbergia odorifera contains high concentrations of flavonoid aglycones and trace flavonoid glycosides. In this study, trace flavonoid glycosides were separated from D. odorifera by titania with matrix solid-phase dispersion (MSPD). Before the MSPD experiment, four standards, including two isoflavone glycosides (genistin and formononetin-8-C-apiosyl (1-6)-glucoside) and their aglycones (genistein and formononetin), were used to compare their retention on a titania column. The effect of acetonitrile concentration and pH on their retention was investigated and a conclusion was drawn that high acetonitrile concentration and pH lead to the greatest difference in the retention of flavonoid as glycosides and aglycones. Besides hydrophilic interaction and ligand-exchange interaction may exist between sugar moiety of flavonoid glycoside and titania, so that flavonoid glycosides have stronger retention than that of aglycones. Based on the chromatographic rule of flavonoid as glycosides and aglycones on the titania column, the MSPD method was optimized to elute high concentration flavonoid aglycones first with 90% acetonitrile and 10% water containing 100 mM ammonium acetate buffer, and then to elute trace flavonoid glycosides with 20% acetonitrile and 80% water containing 1% trifluoroacetate (TFA). Isolated flavonoid glycosides were further analyzed by UPLC-MS/MS, and their fragmentation in MS2 showed they are C-glycosyl flavonoids. ? 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.