文献类型：期刊文献

英文题名：Heterologous gshF gene expression in various vector systems in Escherichia coli for enhanced glutathione production

作者：Wang, Cheng[1];Zhang, Jing[1];Wu, Hui[1];Li, Zhimin[1,2];Ye, Qin[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg Technol, Shanghai 200237, Peoples R China

年份：2015

卷号：214

起止页码：63

外文期刊名：JOURNAL OF BIOTECHNOLOGY

收录：;EI(收录号：20153901316466);WOS:【SCI-EXPANDED(收录号:WOS:000365364900013)】；

基金：This study was supported by the National Science Foundation for Young Scientist of China (Grant No. 21406065), the Fundamental Research Funds for the Central Universities (Grant No. 222201313007 and 22A201514042) and Shanghai Committee of Science and Technology (Grant No. 13DZ1930202).

语种：英文

外文关键词：Antioxidant; Glutathione; Escherichia coil; Bifunctional glutathione synthetase; Fed-batch fermentation

摘要：Glutathione (GSH), an important bioactive product, is widely used in production of pharmaceuticals and foods. In this study, four different vector systems, pET28a, pUC18, pUC19-P32, and pUC19-Pabb, were applied for expression of gshF, encoding the bifunctional glutathione synthetase of Streptococcus thermophiles. These four constructs were named as pET28a-gshF, pUC18-gshF, pUC19-P32-gshF and pUC19-Pabb-gshF, respectively, and then introduced into Escherichia coli strain BL21(DE3) for further investigation of protein expression and GSH production. The expression levels of the GshF in BL21(pUC19-P32-gshF) and BL21(pUC19-Pabb-gshF) were much lower than those of BL21(pET28a-gshF) and BL21(pUC18-gshF). In the fed-batch fermentation, the GSH accumulated by BL21(pUC18-gshF) reached 15.21 g/L, which was the highest level of GSH biosynthesis ever reported. Although BL21(pUC19-Pabb-gshF) produced less GSH compared to BL21(pUC18-gshF), the final GSH concentration produced by BL21 (pUC19-Pabb-gshF) still accumulated to 5.09 g/L, which indicated the potential application of the constitutive promoter in GSH production. (C) 2015 Elsevier B.V. All rights reserved.