文献类型：期刊文献

英文题名：Enhanced 1,3-propanediol production by supply of organic acids and repeated fed-batch culture

作者：Xue, Xuedong[1];Li, Wei[1];Li, Zhimin[1];Xia, Yuelan[1];Ye, Qin[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2010

卷号：37

期号：7

起止页码：681

外文期刊名：JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY

收录：;EI(收录号：20242716576703);WOS:【SCI-EXPANDED(收录号:WOS:000278835500004)】；

基金：This work was supported by BG2006036, the Department of Science and Technology, Jiangsu Province, China, and partly supported by Shanghai Leading Academic Discipline Project, no. B505.

语种：英文

外文关键词：1,3-Propanediol; Organic acid mixture; Repeated fed-batch culture; Klebsiella pneumoniae

摘要：In fed-batch culture of Klebsiella pneumoniae, 1,3-propanediol production was growth associated, while the by-products, including lactic acid and ethanol, increased sharply as the cells grew slowly. When the fed-batch culture was supplied with a mixture of organic acids including citrate, fumarate and succinate, cell growth and 1,3-propanediol production increased significantly, whereas the by-products, especially lactic acid and ethanol, decreased sharply. High concentrations of PDO and acetate inhibited cell growth and PDO production. To improve the PDO production, repeated fed-batch culture with addition of the organic acid mixture was performed in a 5-l reactor. The fed-batch culture was repeated five times, and the 1,3-propanediol yield and concentration reached above 0.61 mol mol(-1) and 66 g l(-1), respectively, in 20 h for each cycle. Furthermore, the PDO productivity reached above 3.30 g l(-1) h(-1) in each cycle, which was much higher than that of the original fed-batch culture.