文献类型：期刊文献

英文题名：Characterization of Inulin Hydrolyzing Enzyme(s) in Oleaginous Yeast Trichosporon cutaneum in Consolidated Bioprocessing of Microbial Lipid Fermentation

作者：Wang, Juan[1];Zhang, Huizhan[1];Bao, Jie[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2015

卷号：177

期号：5

起止页码：1083

外文期刊名：APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

收录：;EI(收录号：20153501212197);WOS:【SCI-EXPANDED(收录号:WOS:000364520100007)】；

基金：This research was supported by the National Basic Research Program of China (2011CB707406) and the National High-Tech Program of China (2012AA022301, 2014AA021901).

语种：英文

外文关键词：Inulin hydrolyzing enzyme; Trichosporon cutaneum; Dry cell weight; Lipid production; Consolidated bioprocessing

摘要：Oleaginous yeast Trichosporon cutaneum CGMCC 2.1374 was found to utilize inulin directly for microbial lipid fermentation without a hydrolysis step. The potential inulinase-like enzyme(s) in T. cutaneum CGMCC 2.1374 were characterized and compared with other inulinase enzymes produced by varied yeast strains. The consolidated bioprocessing (CBP) for lipid accumulated using inulin was optimized with 4.79 g/L of lipid produced from 50 g/L inulin with the lipid content of 33.6 % in dry cells. The molecular weight of the enzyme was measured which was close to invertase in Saccharomyces cerevisiae. The study provided information for inulin hydrolyzing enzyme(s) in oleaginous yeasts, as well as a preliminary CBP process for lipid production from inulin feedstock.