文献类型：期刊文献

英文题名：Systematic Identification of Intracellular-Translocated Candidate Effectors in Edwardsiella piscicida

作者：Zhang, Lingzhi[1];Jiang, Zhiwei[1];Fang, Shan[1];Huang, Yajun[1];Yang, Dahai[1];Wang, Qiyao[1,2,3];Zhang, Yuanxing[1,2,3];Liu, Qin[1,2,3,4]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai, Peoples R China;[3]Shanghai Collaborat Innovat Ctr Biomfg Technol, Shanghai, Peoples R China;[4]Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China

年份：2018

卷号：8

外文期刊名：FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000425302800001)】；

基金：We thank Ka Yin Leung from Trinity Western University for helpful discussions and suggestions. This work was supported by the NSFC grants 31430090 to YZ, 31472308 to QL and 31622059 to QL.

语种：英文

外文关键词：effector; Edwardsiella piscicida; T3SS; T6SS; OMV

摘要：Many bacterial pathogens inject effectors directly into host cells to target a variety of host cellular processes and promote bacterial dissemination and survival. Identifying the bacterial effectors and elucidating their functions are central to understanding the molecular pathogenesis of these pathogens. Edwardsiella piscicida is a pathogen with a wide host range, and very few of its effectors have been identified to date. Here, based on the genes significantly regulated by macrophage infection, we identified 25 intracellular translocation-positive candidate effectors, including all five previously reported effectors, namely EseG, EseJ, EseH, EseK, and EvpP. A subsequent secretion analysis revealed diverse secretion patterns for the 25 effector candidates, suggesting that multiple transport pathways were involved in the internalization of these candidate effectors. Further, we identified two novel type VI secretion system (T6SS) putative effectors and three outer membrane vesicles (OMV)-dependent putative effectors among the candidate effectors described above, and further analyzed their contribution to bacterial virulence in a zebrafish model. This work demonstrates an effective approach for screening bacterial effectors and expands the effectors repertoire in E. piscicida.