文献类型：期刊文献

英文题名：Development of an adsorption procedure for the direct separation and purification of prodigiosin from culture broth

作者：Wang, XD; Tao, JL; Wei, DZ; Shen, YL; Tong, WY

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, New World Inst Biotechnol, Shanghai 200237, Peoples R China

年份：2004

卷号：40

起止页码：277

外文期刊名：BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000225622100008)】；

语种：英文

外文关键词：adsorption resin; chromatography; fermentation; in situ separation; prodigiosin; Serratia marcescens

摘要：A novel procedure for the direct separation and purification of prodigiosin based on adsorption chromatography is provided. A surfactant (Tween 80; 0.1 %, w/v) was used to improve prodigiosin release from the cell envelope. An adsorbent (X-5 resin) with high loading capacity (7.3 mg/g) was selected for the direct recovery of prodigiosin from culture broth by static adsorption or in situ separation. This technology not only simplified the separation process, but also made the recovery yield (83 %) much higher when compared with the conventional method (50 %). As a result, it will be particularly suited for large-scale separation and purification of prodigiosin.