文献类型：期刊文献

英文题名：Oriented assembly of hydrophilic nanochains modified by porous zirconium-based coordination polymers for glycopeptides analysis

作者：Gu, Qinying[1];Zhao, Hongli[1];Zhu, Tianyi[1];Lu, Yichen[1];Lin, Yunfan[1];Yuan, Huihui[1];Lan, Minbo[1,2]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Res Ctr Anal & Test, Shanghai 200237, Peoples R China

年份：2024

卷号：267

外文期刊名：TALANTA

收录：;EI(收录号：20233714710252);WOS:【SCI-EXPANDED(收录号:WOS:001107128900001)】；

基金：This work was supported by the Science and Technology Commission of Shanghai Municipality (STCSM, 20392002300, 21JM0010400) ; and the Fundamental Research Funds for the Central Universities (JKJ01231717) .

语种：英文

外文关键词：Oriented assembly; One-dimensional magnetic nanochains; Porous coordination polymer; Hydrophilicity; Glycopeptide enrichment

摘要：Mass spectrometry (MS)-based glycoproteomics research requires additional sample pretreatment to improve the effective identification of low-abundance glycopeptides without interference from non-glycoproteins. Herein, an attractive strategy using resorcinol-formaldehyde (RF) resin and zirconium-based coordination polymer (Zr-BCP) was established to prepare one-dimensional porous coordination polymer composites for glycopeptide enrichment before MS analysis. The obtained Fe3O4@RF@Zr-BCP nanochains feature excellent magnetic response (42.26 emu/g), high hydrophilicity (16.0 degrees), and large specific surface area (140.84 m2/g), which provides abundant affinity sites for specific capture of glycopeptides. The materials exhibit outstanding performance in the enrichment of glycopeptides in terms of sensitivity (15 fmol/mu L IgG), selectivity (1:200, molar ratio of IgG/ BSA), loading capacity (200 mg/g) and recovery (106.4 +/- 3.5%). In addition, the developed method based on Fe3O4@RF@Zr-BCP has been successfully applied to capture glycopeptides in tryptic digest of mouse teratoma cell extracts. It is worth emphasizing that compared with dispersed nanoparticles, the one-dimensional chain structure brings extraordinary reusability to Fe3O4@RF@Zr-BCP nanochains, which is conducive to the rapid cyclic enrichment of glycopeptides. This present work provides a potential enrichment platform for comprehensive glycoprotein analysis, and opens a new avenue for the application of oriented-assembly nanochains.