文献类型：期刊文献

英文题名：Optimization of cellulase mixture for efficient hydrolysis of steam-exploded corn stover by statistically designed experiments

作者：Zhou, Jin[1];Wang, Yong-Hong[1];Chu, Ju[1];Luo, Ling-Zhi[1];Zhuang, Ying-Ping[1];Zhang, Si-Liang[1]

机构：[1]E China Univ Sci & Technol, Natl Engn Res Ctr Biotechnol Shanghai, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2009

卷号：100

期号：2

起止页码：819

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20084211646522);WOS:【SCI-EXPANDED(收录号:WOS:000261080700047)】；

基金：This work was supported by the National Basic Research Program (973 Program, No. 2007CB714306) and Shanghai Leading Academic Discipline Project (Project No. B505).

语种：英文

外文关键词：Cellulase mixture; Optimization; Steam-exploded corn stover; Statistically designed experiments; Bioethanol

摘要：To improve the enzymatic hydrolytic efficiency and reduce production cost, a statistically designed experimental approach was used to optimize the composition of cellulase mixture so as to maximize the amount of glucose produced from steam-exploded corn stover (SECS). Using seven purified enzymes (cellobiohydrolases, Cel7A, Cel6A,Cel6B: endoglucanases, Cel7B, Cel12A, Cel61A; and beta-glucosidase) from Trichoderma viride T 100-14 mutant strain, a multi-enzyme mixture was constituted after screening and optimization. The final optimal composition (mol%) of the multi-enzyme mixture was Cel7A (19.8%), Cel6A (37.5%), Cel6B (4.7%), Cel7B (17.7%), Cel12A (15.2%), Cel61A (2.3%) and beta-glucosidase (2.8%). The subsequent verification experiments followed by glucose assay together with scanning electron microscopy (SEM) observation confirmed the validity of the models. The multi-enzyme mixture displayed a high performance in converting the cellulosic substrate (SECS). The amount of glucose produced (15.5 mg/ml) was 2.1 times as that of the crude cellulase preparation. The results indicated that the optimized cellulase mixture is an available and efficient paradigm for the hydrolysis of lignocellulosic substrate. The enhanced cellulolytic activity displayed by the constructed cellulase mixture could be used as an effective tool for producing bioethanol efficiently from cellulose. (C) 2008 Elsevier Ltd. All rights reserved.