文献类型：期刊文献

中文题名：静息培养基的筛选及在此体系中庆大霉素的合成

英文题名：Screening Resting Cell System for Study Gentamicin Biosynthesis

作者：谌颉[1];储炬[1];张嗣良[1];李友荣[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2001

卷号：27

期号：2

起止页码：135

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金资助项目 !(3 9870 0 18)

语种：中文

中文关键词：庆大霉素;静息细胞;培养基;筛选;合成

外文关键词：gentamicin; resting cell; culture medium; screening; synthesis

摘要：通过对比实验 ,讨论了培养基中各种组分对庆大霉素合成与分泌的影响 ;由此确定了静息细胞培养基的配比 ,并应用此系统考察了影响庆大霉素合成的因素。结果表明 :培养温度为 34°C、添加适量次级代谢物、体系内硫酸锰浓度为 50 mg/L及每升添加 0 .0 2 0 m L的 Tween2
Through comparative experiments, the concentration of each ingredient in the resting cell medium was determined, and their influences on the synthesis and secretion of gentamicin(GM) were also discussed. Some factors affecting gentamicin biosynthesis were studied by using this system. The results showed that the biosynthesis of GM was promoted when the temperature was controlled at 34°C and some secondary metabolites, 50mg/L MnSO 4 or 0.02mL Tween20 per liter were supplemented.