文献类型：期刊文献

英文题名：Characterization of polycyclic aromatic hydrocarbons degradation and arsenate reduction by a versatile Pseudomonas isolate

作者：Feng, Tiancai[1];Lin, Hanping[1];Tang, Junzhi[1];Feng, Yaoyu[1]

机构：[1]E China Univ Sci & Technol, Sch Resources & Environm Engn, State Environm Protect Key Lab Environm Risk Asse, Shanghai 200237, Peoples R China

年份：2014

卷号：90

起止页码：79

外文期刊名：INTERNATIONAL BIODETERIORATION & BIODEGRADATION

收录：;EI(收录号：20141217484154);WOS:【SCI-EXPANDED(收录号:WOS:000335872000012)】；

基金：This work was supported by the National Special Fund for State Key Laboratory of Bioreactor Engineering (No.2060204) and the Fundamental Research Funds for the Central Universities, China.

语种：英文

外文关键词：Co-contamination; PAHs; Biodegradation; Arsenic; Arsenate reduction; Pteris vittata L

摘要：A Pseudomonas isolate, designated PAHAs-1, was found capable of reducing arsenate and degrading polycyclic aromatic hydrocarbons (PAHs) independently and simultaneously. This isolate completely reduced 1.5 mM arsenate within 48 h and removed approximately 100% and 50% of 60 mg l(-1) phenanthrene and 20 mg l-1 pyrene within 60 h, respectively. Using PAHs as the sole carbon source, however, this isolate showed a slow arsenate reduction rate (4.62 mu M h(-1)). The presence of arsenic affected cell growth and concurrent PAHs removal, depending on PAH species and arsenic concentration. Adding sodium lactate to the medium greatly enhanced the arsenate reduction and pyrene metabolism. The presence of the alpha subunit of the aromatic ring-hydroxylating dioxygenase (ARHD) gene, arsenate reductase (arsC) and arsenite transporter (ACR3(2)) genes supported the dual function of the isolate. The finding of latter two genes indicated that PAHAs-1 possibly reduced arsenate via the known detoxification mechanism. Preliminary data from hydroponic experiment showed that PAHAs-1 degraded the majority of phenanthrene (>60%) and enhanced arsenic uptake by Pteris vittata L (from 246.7 to 1187.4 mg kg(-1) As in the fronds). The versatile isolate PAHAs-1 may have potentials in improving the bioremediation of PAHs and arsenic co-contamination using the plant-microbe integrated strategy. (C) 2014 Elsevier Ltd. All rights reserved.