文献类型：期刊文献

英文题名：Polyetheretherketone fibers woven fabrics coated nanostructured silicon nitride and load EGCG as artificial ligaments for promoting ligament-bone integration

作者：Wang, Fan[1];Xie, En[1];Wang, Xuehong[1];Sun, Ping[2];Ji, Yinjun[3];Niu, Yunfei[3];Wang, Dongliang[4];Li, Fengqian[2];Wei, Jie[1]

机构：[1]East China Univ Sci & Technol, Key Lab Ultrafine Mat, Minist Educ, Shanghai 200237, Peoples R China;[2]Shanghai Eighth Peoples Hosp, Shanghai 200235, Peoples R China;[3]Second Mil Med Univ, Changhai Hosp, Dept Orthoped, Shanghai 200433, Peoples R China;[4]Shanghai Jiao Tong Univ, Xin Hua Hosp, Dept Orthoped Surg, Sch Med, 1665 Kongjiang Rd, Shanghai 200092, Peoples R China

年份：2022

卷号：27

外文期刊名：APPLIED MATERIALS TODAY

收录：;EI(收录号：20220711649703);WOS:【SCI-EXPANDED(收录号:WOS:000783940500009)】；

基金：Acknowledgements The grants were from the National Natural Science Founda-tion of China (81772343, 32171340 and 81971753) and Key Med-ical Program of Science and Technology Development of Shanghai (19441906100 and 20S31900400) and National Science Foundation of Shanghai (21ZR1447900) .

语种：英文

外文关键词：PEEK fiber; Artificial ligament; Macrophage polarization; Immunomodulatory; ligament -bone integration

摘要：Design of an artificial ligament with osteogenic activity to promote ligament-bone integration for an-terior cruciate ligament (ACL) reconstruction remains a huge challenge. In this work, polyetheretherke-tone fibers with nanostructured silicon nitride coating (PSN) and loading epigallocatechin gallate (EGCG) were woven into fabrics (PSN@EG) for artificial ligament application.The in vitro experiments confirmed that PSN@EG significantly upregulated M2 macrophage polarization that promoted secretory of anti-inflammatory cytokines while downregulated M1 macrophage polarization that inhibithed secretory of pro-inflammatory cytokines. Moreover, PSN@EG remarkably improved osteoblastic differentiation of rat bone marrow mesenchymal stem cells (BMSC) while suppressed osteoclastic differentiation of rat bone marrow-derived macrophages (BMM) in macrophage condition medium. The in vivo experiments revealed that PSN@EG promoted new bone formation for ligament-bone integration that inhibited fibrous encap-sulation formation. It could be suggested that the synergistic effects of the optimized surface charactieris-tics (e.g., nano-micro topography), and sustained release of Si ions and EGCG caused the immunomod-ulatory of PSN@EG, which improved pro-osteogenic macrophage polarization that provided a favorable anti-inflammatory immune environment for enhancing osteogenesis while inhibiting osteoclastogenesis. In summary, PSN@EG with immunomodulatory significantly promoted osteoblastic differentiation while suppressed osteoclastic differentiation, thereby facilitating osteogenesis for ligament-bone integration. As a novel artificial ligament, PSN@EG has tremendous potential for ACL reconstruction.(c) 2022 Elsevier Ltd. All rights reserved.