文献类型：期刊文献

英文题名：Development of an engineered thermostable amine dehydrogenase for the synthesis of structurally diverse chiral amines

作者：Liu, Lei[1];Wang, Dong-Hao[1];Chen, Fei-Fei[1];Zhang, Zhi-Jun[1];Chen, Qi[1];Xu, Jian-He[1];Wang, Zhi-Long[2];Zheng, Gao-Wei[1]

机构：[1]East China Univ Sci & Technol, Shanghai Collaborat Innovat Ctr Biomfg, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Jiao Tong Univ, Sch Pharm, State Key Lab Microbial Metab, Shanghai 200240, Peoples R China

年份：2020

卷号：10

期号：8

起止页码：2353

外文期刊名：CATALYSIS SCIENCE & TECHNOLOGY

收录：;EI(收录号：20202008644310);WOS:【SCI-EXPANDED(收录号:WOS:000530288800034)】；

基金：This work was financially supported by the National Natural Science Foundation of China (No. 21878085, 21536004 and 21871085), the Fundamental Research Funds for the Central Universities (22221818014), and the State Key Laboratory of Microbial Metabolism (MMLKF18-09).

语种：英文

外文关键词：Ammonia - Amino acids - Amines

摘要：Amine dehydrogenases (AmDHs) are emerging as a class of attractive biocatalysts for synthesizing chiral amines via asymmetric reductive amination of ketones with inexpensive ammonia as an amino donor. However, the AmDHs developed to date exhibit limited substrate scope. Here, using directed evolution, we engineered a GkAmDH based on a thermostable phenylalanine dehydrogenase from Geobacillus kaustophilus. The newly developed AmDH is able to catalyze reductive amination of a diverse set of ketones and functionalized hydroxy ketones with ammonia or primary amines with up to >99% conversion, thus accessing structurally diverse chiral primary and secondary amines and chiral vicinal amino alcohols, with excellent enantioselectivity (up to >99% ee) and releasing water as the sole by-product.