文献类型：期刊文献

中文题名：固定化脂肪酶的动力学研究

英文题名：Study on the Kinetics of Immobilized Lipase

作者：李志国[1];欧伶[1];宋庆训[1];魏东芝[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室生物化学研究所,上海200237

年份：2004

卷号：30

期号：1

起止页码：107

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;EI(收录号：2004138091833);Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

基金：上海市重点学科资助项目

语种：中文

中文关键词：脂肪酶;固定化;壳聚糖;物理吸附;动力学

外文关键词：lipase; immobilization; chitosan; physical adsorption; kinetics

摘要：以壳聚糖为载体,用物理吸附固定化脂肪酶,对影响固定化过程的各种因素进行了考察,确定了最优条件,并比较了游离酶和固定化酶的最适温度、pH、酶的热稳定性以及表观Km。固定化酶水解的最适pH(pH7.0)稍低于游离酶(pH8.0),最适温度为50°C(游离酶为40°C)。固定化酶在50°C的半衰期(0.96h)是游离酶(0.32h)的3倍,在4°C的冰箱中贮存3个月活力变化很小。
Lipase (EC3.1.1.3) from Candida sp. was immobilized on chitosan by physical adsorption. In this article, different factors that influenced the immobilization were investigated, and the optimum conditions were ascertained. Comparative studies of pH, temperature and thermal stability between free lipase and immobilized lipase were conducted. As a result, the optimum pH(7.0) of the immobilized lipase was lower than that of the soluble lipase (8.0). The optimum reaction temperature shifted from 40(°C) for the free lipase to 50(°C) for the immobilized lipase. The patterns of thermal stability indicated that the immobilization process tends to stabilize the enzyme. Compared with the half-life(0.32 h) of the soluble free (lipase) at 50(°C), the half-life(0.96 h) of the immobilized lipase increased three times. The activity of immobilized lipase changed slightly after 3 months storage at 4(°C).