文献类型：期刊文献

英文题名：A novel method of prediction and optimization for preparative high-performance liquid chromatography separation

作者：Jin, Yu[1];Xue, Xingya[1];Liu, Yanfang[1];Xiao, Yuansheng[1];Zhang, Jing[1];Shi, Hui[2];Zhang, Feifang[1];Liang, Xinmiao[1,2]

机构：[1]Chinese Acad Sci, Grad Sch, Dalian Inst Chem Phys, Dalian 116023, Peoples R China;[2]E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China

年份：2008

卷号：1183

期号：1-2

起止页码：76

外文期刊名：JOURNAL OF CHROMATOGRAPHY A

收录：;EI(收录号：20080711099008);WOS:【SCI-EXPANDED(收录号:WOS:000253869900009)】；

语种：英文

外文关键词：prediction; optimization; preparative high-performance liquid chromatography (preparative HPLC); retention equation; complex sample; traditional Chinese medicine (TCM)

摘要：The preparation of components from a complex sample is a difficult task. The optimization of the separation and subsequent scale-up is usually carried out by trial and error. In this study, the relationship between retention parameters a and c for analytical and preparative separations was developed when the same solid adsorbent and mobile phase were used. The prediction and optimization of the preparative separation of a complex sample could be achieved by direct and simple conversion of the experimentally determined data from an analytical level. The novel method was successfully applied to optimize the separation of target compounds in traditional Chinese medicine (TCM). (C) 2008 Elsevier B.V. All rights reserved.