文献类型：期刊文献

英文题名：Accurate Determination of Isotopic Abundance of Intracellular Metabolites of Saccharopolysporaerythraea Based on Ultra Performance Liquid Chromatography-Triple Quadrupole Mass Spectrometry

作者：Mou Han[1];Hong Ming[1];Liu Xiao-Yun[1];Li Min-Chao[1];Huang Ming-Zhi[1];Chu Ju[1];Zhuang Ying-Ping[1];Zhang Si-Liang[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2017

卷号：45

期号：9

起止页码：1264

外文期刊名：CHINESE JOURNAL OF ANALYTICAL CHEMISTRY

收录：;EI(收录号：20173704155325);WOS:【SCI-EXPANDED(收录号:WOS:000410913700002)】；

基金：This work was supported by the National Natural Science Foundation of China (No. 21276081), and the National Science and Technology Support Project of China (No. 2011BAF02B05).

语种：英文

外文关键词：Saccharopolysporaerythraea; Intracellular metabolites; C-13 isotopic abundance; Ultra-high performance liquid chromatography-triple quadrupole mass spectrometry

摘要：A method was established for measuring C-13 isotopic abundance of intracellular metabolites of Saccharopolysporaerythraea by ultra-high performance liquid chromatography (UPLC)-triple quadrupole mass spectrometry. Under the optimized chromatographic conditions of UPLC, and the appropriate and unique tube lens voltage, collision energy and ion pair, one-to-one method, one-to-many method and SIM method were established for measuring C-13 isotopic abundance based on the length of the parent and daughter ions carbon chains and whether the daughter ions contain C-13 atoms. The three methods were then applied to measure naturally labeled intracellular metabolite standards and C-13 labeled samples. According to the gap between the experimental value and the theoretical value, the best method was found for each metabolite of different characteristics. The results showed that one-to-one method was the most effective for measuring the metabolites of daughter ions not containing C-13 atoms represented by sugar phosphates, one-to-many method was the best for measuring the metabolites of both parent and daughter ions containing C-13 short carbon chains represented by carboxylic acids, SIM method could played a role in measuring the metabolites of both parent and daughter ions containing C-13 long carbon chains represented by coenzyme A. These methods had a good measurement precision and could be applied to the measurement of Saccharopolysporaerythraea intracellular metabolites, which would contribute to consequent study of the metabolic mechanism and the efficient expression of erythromycin.