文献类型：期刊文献

中文题名：SAM产生菌酿酒酵母HYS98的发酵方式

英文题名：Fermentation Modes of Saccharomyces cerevisiae HYS98 for High Productivity of S-adenosyl-L-methionine

作者：沈剑[1];李元广[1];沈国敏[1];杨东[2]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237;[2]上海泽元海洋生物技术有限公司,上海200237

年份：2008

卷号：8

期号：3

起止页码：561

中文期刊名：过程工程学报

外文期刊名：The Chinese Journal of Process Engineering

收录：CSTPCD;;EI(收录号：20082911382851);Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：国家十五科技攻关基金资助项目(编号:2004BA713B01-07)

语种：中文

中文关键词：酿酒酵母HYS98;S-腺苷甲硫氨酸;发酵方式

外文关键词：Saccharomyces cerevisiae HYS98; S-adenosyl-L-methionine; fermentation mode

摘要：分别研究了分批发酵、恒速流加发酵、变速流加发酵和分批补料发酵4种操作方式下酿酒酵母HYS98发酵生产SAM的代谢特征,并考察了不同发酵方式对发酵结果的影响.结果表明,酿酒酵母HYS98发酵生产SAM的最佳发酵模式为分批补料发酵,SAM的平均产率和菌体生长速率分别达0.123和1.030g/(L·h),比国内外文献报道的最高水平分别提高了68%和73%,为发酵工艺进一步优化奠定了基础.
The metabolic characteristics under four operation ways of batch fermentation, constant feeding rate fermentation, changing feeding rate fermentation and batch feeding fermentation, were studied in a 5 L fermentator. And the influences of different S-adenosyl-L-methionine （SAM） fermentation modes were investigated. The optimum fermentation mode among them of Saccharornyces cerevisiae HYS98 was batch feeding. The average productivity and cell growth rate of the feed batch fermentation were obtained as 0.123 g/（L-h） and 1.030 g/（L.h）, which were 68% and 73% higher than the highest levels reported in the literature respectively. The results laid the foundations for fixrther optimization of SAM fermentation process.