文献类型：期刊文献

英文题名：Enhanced succinate production from glycerol by engineered Escherichia coli strains

作者：Li, Qing[1];Wu, Hui[1];Li, Zhimin[1,2];Ye, Qin[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg Technol, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2016

卷号：218

起止页码：217

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20162702558393);WOS:【SCI-EXPANDED(收录号:WOS:000384710500031)】；

基金：This work was supported by the National High Technology Research and Development Program of China (No. 2011AA02A203), the National Science Foundation for Young Scientist of China (Grant No. 21406065), the Fundamental Research Funds for the Central Universities (Grant Nos. 222201313007 & 22A201514042). Partially supported by Open Funding Project of the State Key Laboratory of Bioreactor Engineering. Partially supported by the Foundation of Key Laboratory for Industrial Biocatalysis (Tsinghua University), Ministry of Education (Grant No. 2015101).

语种：英文

外文关键词：Succinate; Escherichia coli; Glycerol; Phosphoenolpyruvate carboxykinase; Two-stage fermentation

摘要：In this study, an engineered strain Escherichia coli MLB (ldhA(-) pflB(-)) was constructed for production of succinate from glycerol. The succinate yield was 0.37 mol/mol in anaerobic culture, however, the growth and glycerol consumption rates were very slow, resulting in a low succinate level. Two-stage fermentation was performed in flasks, and the succinate yield reached 0.93 mol/mol, but the succinate titer was still low. Hence, overexpression of malate dehydrogenase, malic enzyme, phosphoenolpyruvate (PEP) carboxylase and PEP carboxykinase (PCK) from E. coli, and pyruvate carboxylase from Corynebacterium glutamicum in MLB was investigated for improving succinate production. Overexpression of PCK resulted in remarkable enhancement of glycerol consumption and succinate production. In flask experiments, the succinate concentration reached 118.1 mM, and in a 1.5-L bioreactor the succinate concentration further increased to 360.2 mM. The highest succinate yield achieved 0.93 mol/mol, which was 93% of the theoretical yield, in the anaerobic stage. (C) 2016 Elsevier Ltd. All rights reserved.