文献类型：期刊文献

中文题名：大肠杆菌乙酸代谢突变株的选育和特性研究

英文题名：SCREENING AND CHARACTERISTICS OF MUTANTS OF E. COLI RESISTANT TO ACETATE INHIBITION

作者：李志敏[1];叶勤[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2001

卷号：41

期号：2

起止页码：223

中文期刊名：微生物学报

外文期刊名：Acta Microbiologica Sinica

收录：CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；PubMed;

语种：中文

中文关键词：大肠杆菌;诱变选育;连续培养;乙酸;代谢突变株

外文关键词：Escherichia coli, Mutagenesis, Continuous culture, Acetate

摘要：在大肠杆菌高密度培养中 ,因代谢副产物乙酸积累 ,导致抑制菌体的生长和产物表达的下降。为减小乙酸的抑制作用 ,采用60 Co诱变处理大肠杆菌JM1 0 1 ,结合连续培养 (含乙酸钠选择压力 )定向富集方法 ,选育到一株乙酸耐受性增强的菌株JL3。该菌株表现出明显的乙酸耐受性的提高 ,在含有 1 0 g/L乙酸钠的MA培养基中 ,菌体生长和葡萄糖消耗速率都有较大程度提高 。
Acetate is accumulated in the aerobic high cell density culture of Escherichia coli ,Which seriously inhibits cell growth and expression of recombinant proteins.To alleviate acetate inhibition,mutants of E.coli JM101 were generated by 60 Co radiation and then enriched in continuous culture with acetate as the selective pressure.One of the mutants isolated,JL3,showed obvious increased tolerance toward acetate and maintained phenotypic stability on slant without acetate.In MA medium containing 10g/L of sodium acetate,the specific growth rate and the glucose consumption rate of JL3 were higher than those of JM101.