文献类型：期刊文献

英文题名：Transcriptome-based global analysis of gene expression in response to carbon dioxide deprivation in the green algae Chlorella pyrenoidosa

作者：Fan, Jianhua[1];Xu, Hui[1];Li, Yuanguang[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2016

卷号：16

起止页码：12

外文期刊名：ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000375610000002)】；

基金：The authors appreciate the assistance from Personalbio (Shanghai, China) for sequencing, assembly and annotation. This work was supported by National Natural Science Foundation of China 31300295 and 21306222, "Chenguang Program" by Shanghai Education Development Foundation and Shanghai Municipal Education Commission 14CG27, National Special Fund for State Key Laboratory of Bioreactor Engineering 2060204, National Basic Research Program of China 2011CB200904, the Fundamental Research Funds for the Central Universities WF1314033, and Open Funding Project of the State Key Laboratory of Bioreactor Engineering 2060204.

语种：英文

外文关键词：Chlorella; CO2; Growth; Carbon concentrating mechanism; Gene expression

摘要：Microalgae can tolerate different CO2 concentrations and its mechanism of rapid CO2 fixation is key to understanding the relationship between intracellular substance and energy conversion, as well as to optimize the production of microalgae bioproducts. The present study conducted a transcriptome-based analysis of gene expression of an industrial Chlorella pyrenoidosa strain, FACHB-9, with high oil production ability under different CO2 concentrations. Transcriptome-based gene expression analysis indicated the redirection of central metabolism under CO2 deprivation. The C-3 pathway served as the main metabolic pathway for C. pyrenoidosa, which was subjected to a high CO2 environment in the present study. Similar to C-4 plants wherein limited CO2 activates CO2-concentrating mechanism to compensate for the low activity of RuBisCO in the Calvin cycle, C. pyrenoidosa undergoes CO2 compensation by active transport to ensure sufficient amounts of Ci for its growth and metabolism. Comparative analysis has allowed the identification of several candidate genes for further strain improvement. These genes encode proteins that might be CAs, as indicated by its localization to the cell membrane or chloroplast membrane, or act as Ci transporters that assist Ci transmembrane transportation. Certain types of ABC transport proteins and CCCH-type zinc finger proteins also showed significant changes in gene expression. These findings indicate that these may be promising targets for functional and genetic modification studies. The results not only reveal the significance of the mechanism of carbon sequestration to eukaryotic green algae, but also provide a basis for further construction of the energy microalgae cell factory with high efficiency carbon biofixation capacity. (C) 2016 Elsevier B.V. All rights reserved.