文献类型：期刊文献

中文题名：人皮肤成纤维细胞在不同培养系统中的生长代谢特性

英文题名：Growth and Metabolism of Human Skin Fibroblasts Cultured on Microcarriers

作者：邓明安[1];周燕[1];华平[1];谭文松[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2001

卷号：17

期号：3

起止页码：336

中文期刊名：生物工程学报

外文期刊名：Chinese Journal of Biotechnology

收录：CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；PubMed;

基金：国家"8 6 3"计划生物领域项目;国家重点基础研究发展规划项目 ( 973)基金资助! ( 10 2 0 9 0 5 0 4;G19990 5 430 9 2 )&&

语种：中文

中文关键词：组织工程;细胞培养;微载体;代谢;皮肤;成纤维细胞

外文关键词：tissue engineering, cell culture, microcarrier, metabolism

摘要：Human foreskin fibroblasts were cultured \%in vitro\% on microcarriers in spinners and traditional static flasks.The cultured cells obtained with these approaches were compared in cell shape,cell growth,cell production,and the metabolisms of glucose, lactate,and ammonium.The cells from microcarrier cultures through medium exchange were 8 times more than those from traditional static flasks.It was found that the specific growth rate and specific glucose consumption rate in microcarrier spinner cultures were 0.64/d -1 and 5.56mmol/10\+9cell/d,respectively,higher than those in static flask cultures.However,the average lactate yield on glucose consumption in spinner cultures was only 0.955mmol/mmol,lower than that in static flask cultures,1.125mmol/mmol.This indicated that the energy metabolism in spinner cultures was significantly more efficient than that in static flasks.The experimental results from this work suggest that the microcarrier culture system is a suitable way to expand the seeding cells for tissue engineering, due to its ideal cultivation environment provided.
Human foreskin fibroblasts were cultured \%in vitro\% on microcarriers in spinners and traditional static flasks.The cultured cells obtained with these approaches were compared in cell shape,cell growth,cell production,and the metabolisms of glucose, lactate,and ammonium.The cells from microcarrier cultures through medium exchange were 8 times more than those from traditional static flasks.It was found that the specific growth rate and specific glucose consumption rate in microcarrier spinner cultures were 0.64/d -1 and 5.56mmol/10\+9cell/d,respectively,higher than those in static flask cultures.However,the average lactate yield on glucose consumption in spinner cultures was only 0.955mmol/mmol,lower than that in static flask cultures,1.125mmol/mmol.This indicated that the energy metabolism in spinner cultures was significantly more efficient than that in static flasks.The experimental results from this work suggest that the microcarrier culture system is a suitable way to expand the seeding cells for tissue engineering, due to its ideal cultivation environment provided.