文献类型：期刊文献

英文题名：Development of a fed-batch process for the production of anticancer drug TATm-survivin(T34A) in Escherichia coli

作者：Zhang, Haiyi[1]; Zheng, Yu[1]; Liu, Qinghai[1]; Tao, Xinyi[1]; Zheng, Wenyun[1]; Ma, Xingyuan[1]; Wei, Dongzhi[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China

年份：2009

卷号：43

期号：2

起止页码：163

外文期刊名：Biochemical Engineering Journal

收录：EI(收录号：20085211809792)

语种：英文

外文关键词：Batch cell culture - Controlled drug delivery - Recombinant proteins - Batch data processing - Gene expression

摘要：A fed-batch process was developed for intracellular production of recombinant TATm-survivin(T34A) in Escherichia coli under the control of T7 promoter. The effects of induction mode and nutritional conditions were investigated. Compared to the one-point addition of inducer, the step-wise addition of isopropyl beta-d-thiogalactopyranoside (IPTG) maintained higher plasmid stability and increased the production level by 52%. Insufficient glucose supply after induction was observed to control acetate accumulation effectively and improved the expression of the target gene evidently. Remarkably, the pre-induction supplement of inorganic nitrogen source had a positive influence on the production of TATm-survivin(T34A). High ammonium concentration of 4.8 g l-1 was the most efficient in enhancing the production level (as the percentage of total cellular protein) and the specific productivity of TATm-survivin(T34A). As a result, the production of TATm-survivin(T34A) was optimized from 11.6% to 36.8% of total cellular protein (corresponding to 1.68 g l(1). The findings provide valuable information for optimization of recombinant protein expression. ? 2008 Elsevier B.V. All rights reserved.