文献类型：期刊文献

英文题名：Disinfection and mechanism of super-resistant Acinetobacter sp. and the plasmid-encoded antibiotic resistance gene blaNDM-1 by UV/ peroxymonosulfate

作者：Yao, Shijie[1];Hu, Yaru[1];Ye, Jianfeng[2];Xie, Jianhao[3];Zhao, Xuetao[4];Liu, Lingli[1];Lyu, Shuguang[1];Lin, Kuangfei[1];Cui, Changzheng[1,5,6]

机构：[1]East China Univ Sci & Technol, Sch Resources & Environm Engn, State Environm Protect Key Lab Environm Risk Asse, Shanghai 200237, Peoples R China;[2]Shanghai Acad Environm Sci, Shanghai 200233, Peoples R China;[3]Fudan Univ, Childrens Hosp, Shanghai 200233, Peoples R China;[4]Ctr Dis Control Prevent Xuhui, Shanghai 200237, Peoples R China;[5]East China Univ Sci Technol, Shanghai Environm Protect Key Lab Environm Standa, Shanghai 200237, Peoples R China;[6]East China Univ Sci Technol, Natl Engn Lab Ind Wastewater Treatment, Shanghai 200237, Peoples R China

年份：2022

卷号：433

外文期刊名：CHEMICAL ENGINEERING JOURNAL

收录：;EI(收录号：20214711188596);WOS:【SCI-EXPANDED(收录号:WOS:000777264400007)】；

基金：Acknowledgements This research was supported by the Science and Technology Com-mission of Shanghai Municipality (No. 20dz1200800) and Shanghai Sailing Program (No. 21YF1409700) .

语种：英文

外文关键词：Super antibiotic-resistant bacteria; Antibiotic resistance genes;

UV/PMS

摘要：The super-resistant bacteria that produce the New Delhi metallo-beta-lactamase 1 (NDM-1) enzyme are becoming a conceivably major worldwide clinical issue with serious consequences. Studying the disinfection and mechanism of super-resistant bacteria and their antibiotic resistance genes (ARGs) is crucial for controlling their spread in the water environment. In this study, the disinfection of the super-resistant bacterium Acinetobacter sp. CS-2 and its transferable ARGs (plasmid-encoded bla(NDM-1) and bla(OXA-58)) by ultraviolet radiation (UV), peroxymonosulfate (PMS), and ultraviolet/peroxymonosulfate (UV/PMS) exposure were investigated. UV/PMS could completely inactivate CS-2 and control the photoreactivation risk, while bacteria that underwent UV and PMS treatment had survival ratios of 0.22% and 0.28%, respectively. The removal efficiencies of bla(NDM-1) and bla(OXA-58) ranged from 3.64 to 4.05 log, and the advantage of UV/PMS treatment on ARGs damage was further identified by gel electrophoresis analysis. The removal efficiency of bla(NDM-1) was lower than that of bla(OXA-58), which was attributed to the low number of adjacent 5 & PRIME;-TT-3 & PRIME; harbored by blaNDM-1. The removal rate constants of the ARGs by UV treatment were significantly correlated with the number of adjacent base pairs (e.g., 5 & PRIME;-TT-3 & PRIME;, 5 & PRIME;-CT-3 & PRIME;, 5 & PRIME;-TC-3 & PRIME; and 5 & PRIME;-CC-3 & PRIME;) and length, with R-values ranging from 0.9044 to 0.9512. Singlet oxygen (O-1(2)) played a vital role in the reduction of bla(NDM-1) and bla(OXA-58) in the UV/PMS treatment. UV/PMS showed high removal efficiency under natural pH conditions and in the presence of various aqueous ions (e.g., Cl-, SO(4)(2-& nbsp;)and HCO3- ). Overall, this study provides new understandings and theoretical support for the risk control of super-resistant bacteria and plasmid-encoded ARGs.