文献类型：期刊文献

英文题名：Fed-batch culture of hybridoma cells in serum-free medium using an optimized feeding strategy

作者：Zhang, Li[1]; Shen, Hong[1]; Zhang, Yuanxing[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2004

卷号：79

期号：2

起止页码：171

外文期刊名：JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

收录：;EI(收录号：2004088022747);WOS:【SCI-EXPANDED(收录号:WOS:000188500900009)】；

语种：英文

外文关键词：hybridoma cell; serum-free medium; fed-batch culture; kinetic model

摘要：The achievement of high cell density and monoclonal antibody concentration depends on understanding cell metabolism and the control of cell culture. A model of cell metabolism and monoclonal antibody production in hybridoma cell culture is presented. Fed-batch culture in serum-free medium was conducted in a bioreactor with an optimized feeding strategy according to the model. In the process, nutrient concentrations, especially glucose and glutamine, were maintained at relatively constant and low levels. The formation of toxic byproducts, such as ammonia and lactate, was greatly reduced in comparison with that in non-optimized batch culture. The concentration of monoclonal antibody reached 350 mg dm(-3), which was seven times greater than in non-optimized batch culture. (C) 2004 Society of Chemical Industry.