文献类型：期刊文献

英文题名：Molecular diversity of bacterial bamA gene involved in anaerobic degradation of aromatic hydrocarbons in mesophilic petroleum reservoirs

作者：Ruan, Meng-Ya[1,2];Liang, Bo[1,2];Mbadinga, Serge Maurice[1,2,4];Zhou, Lei[1,2];Wang, Li-Ying[1,2];Liu, Jing-Feng[1,2];Gu, Ji-Dong[3];Mu, Bo-Zhong[1,2,4]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Inst Appl Chem, Shanghai, Peoples R China;[3]Univ Hong Kong, Sch Biol Sci, Pokfulam Rd, Hong Kong, Hong Kong, Peoples R China;[4]Shanghai Collaborat Innovat Ctr Biomfg Technol, Shanghai 200237, Peoples R China

年份：2016

卷号：114

起止页码：122

外文期刊名：INTERNATIONAL BIODETERIORATION & BIODEGRADATION

收录：;EI(收录号：20162602548667);WOS:【SCI-EXPANDED(收录号:WOS:000383528000017)】；

基金：This work was supported by the National Natural Science Foundation of China (Grant No. 41530318, 41373070), the NSFC/ RGC Joint Research Fund (Grant No. 41161160560).

语种：英文

外文关键词：bamA gene; Anaerobic aromatic ring cleavage; Mesophilic; Oil reservoir

摘要：Aromatic hydrocarbons are usually metabolized to the central intermediate benzoyl-coenzyme A (CoA) prior to ring cleavage. 6-oxocyclohex-1-ene-1-carbonyl-CoA hydrolase, a key enzyme involved in the reductive benzene ring cleavage, encoded by the bamA gene can serve as a functional biomarker for detecting anaerobic degradation of aromatic compounds in different environments. In the present study, we examined the diversity the bacterial bamA gene in oil field. We sequenced bamA fragments that were PCR amplified from the microbial biomass of two mesophilic oil producing reservoirs during a period of four months. The results showed the presence and a higher diversity of bamA gene than previous studies, indicating its potential involvement in degrading aromatic hydrocarbons through anaerobic biochemical pathways in oil fields. The bamA gene sequences were affiliated to bacteria that are known to degrade aromatics, including Azoarcus, Georgfuchsia, Sulfuritalea, Peptococcaceae and Syntrophus. Differences of composition and diversity of bamA gene sequences over a period of four months in these oil reservoirs showed that it is necessary to monitor functional genes over a period of time to fully establish their significance to any specific process at any site. (C) 2016 Elsevier Ltd. All rights reserved.