文献类型：期刊文献

中文题名：莱鲍迪苷A的制备色谱法分离纯化研究

英文题名：Isolation and purification of Rebaoudioside-A by Medium Pressure Preparative Liquid Chromatography

作者：张凌云[1,2];徐环昕[1,2];刘坐镇[1,2];宁方红[1,2];江邦和[1,2]

机构：[1]华东理工大学生物反应器国家重点实验室分离工程研究室,上海200237;[2]上海华震科技有限公司,上海200237

年份：2013

卷号：34

期号：19

起止页码：216

中文期刊名：食品工业科技

外文期刊名：Science and Technology of Food Industry

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：甜菊糖产业技术企业横向课题

语种：中文

中文关键词：制备色谱;莱鲍迪苷A;甜菊糖结晶母液;改性PS—DVB基球填料

外文关键词：Preparative Liquid Chromatography ； Rebaoudioside- A; Stevia glycosides crystallizing liquid ； ModifiedPS-DVB resin

摘要：本文从制备色谱填料入手,探讨采用中压制备色谱法一次分离得到高纯度莱鲍迪苷A(RA)的可行性。以羧酸改性15μm单分散PS-DVB基球填料为分离介质,装填入规格为12.6×2.66cm的flash色谱柱中,以RA单体的纯度、产率、回收率为考察指标,研究了不同的流动相比例、上样浓度、上样体积以及流动相流速对分离纯化过程的影响。建立了合适的操作条件:流动相为乙腈/水=80/20(v/v),上样浓度为280mg/mL,上样体积为0.4mL,流速为4mL/min。在最佳的操作条件下,分离得到的RA单体纯度为90.22%,回收率79%。该方法具有分离效率高,产品质量好,生产周期短,操作方便等特点,为从低含量甜菊糖结晶母液中纯化RA单体的工业化生产提供了一种有效的途径。
A medium pressure preparative liquid chromatography （MPLC）was used based on a type of packing material to isolate RA of high purity for the first time.The 15μm modified PS-DVB packing material was devised into a flash column（ 12.6 x 2.66cm）.Purity, yield rate and recovery rate were investigated to study the proportion of mobile phase, sample concentration, sample volume as well as the mobile phase flow rate on the separation and purification process of RA.The optimal operating conditions were as follows, the proportion of mobile phase （acetonitrile/water） was 80/20 （ v/v）, sample concentration 280mg/mL, sample volume 0.4mL, and the flow rate 4mL/min.Under the optimal operating conditions, the obtained RA purity was 90.22%, and the recovery rate achieved 79%.This method was proved to be highly efficient for its good product quality, short production cycle, easy operation, providing a favorable reference for the RA industrialization research.