文献类型：期刊文献

英文题名：Synthesis of galacto-oligosaccharides in AOT/isooctane reverse micelles by β-galactosidase

作者：Chen, Shao-Xin[1]; Wei, Dong-Zhi[1]; Hu, Zhen-Hua[1]

机构：[1]E China Univ Sci & Technol, Res Inst Biochem, Shanghai 200237, Peoples R China

年份：2001

卷号：16

期号：2

起止页码：109

外文期刊名：JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

收录：;EI(收录号：2001496756466);WOS:【SCI-EXPANDED(收录号:WOS:000172197400006)】；

语种：英文

外文关键词：galacto-oligosaccharides; beta-galactosidase; AOT/isooctane; reverse micelles

摘要：Synthesis of galacto-oligosaccharides (GOS) from lactose by beta -galactosidase was investigated in ACT/isooctane reverse micelles where the enzyme was fairly stable. GOS yield increased with initial lactose concentration in both reverse micelles and aqueous system. Transgalactosylation reaction of the enzyme was strongly dependent on the molar ratio of water to surfactant (w(0)) of reverse micelles. At pH 7.0 and 45 degreesC, a maximum GOS concentration from 45% (w/v) lactose solution was 51.2% (w/w) in the reverse micelles with w(0) = 15, while a maximum of 31% in aqueous media. Effects of solvents on GOS formation were also examined. (C) 2001 Elsevier Science B.V. All rights reserved.