文献类型：期刊文献

英文题名：Chemical fingerprint and quantitative analysis of Salvia plebeia R.Br. by high-performance liquid chromatography

作者：Jin, Xiao-feng[1];Lu, Yan-hua[1];Wei, Dong-zhi[1];Wang, Zheng-tao[2]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, New World Inst Biotechnol, Shanghai 200237, Peoples R China;[2]Shanghai Univ TCM, Inst Chinese Mat Med, Shanghai 201203, Peoples R China

年份：2008

卷号：48

期号：1

起止页码：100

外文期刊名：JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000259339800014)】；

基金：This work is supported by Shanghai Leading Academic Discipline Project (13505).

语种：英文

外文关键词：Salvia plebeia R.Br.; HPLC; quality control; fingerprint

摘要：To control the quality of Salvia plebeia R.Br., a simple and reliable method of high-performance liquid chromatography coupled with photodiode array detector (HPLC-DAD) was developed both for fingerprint analysis and quantitative determination of seven bioactive compounds, namely caffeic acid, luteolin-7-glucoside, nepetin-7-glucoside, homoplantaginin, luteolin, nepetin and hispidulin. In fingerprint analysis, twelve peaks were selected as characteristic peaks. in quantitative analysis, seven compounds showed good regression (R-2 > 0.9995) within test ranges and the recovery of the method was in the range of 91.7-103.2%. The content ranges (mg/g) of seven compounds in the collected samples of S. plebeia were 0.80-1.67 (hispidulin), 2.18-5.75 (homoplantaginin), 0.52-1.22 (nepetin), 1.56-3.48 (nepetin-7-glucoside), 0.12-0.24 (luteolin), 0.97-2.22 (luteolin-7-glucoside) and 0.21-0.44 (caffeic acid), respectively. From the results obtained, the content of homoplantaginin was the highest. In addition, luteolin and luteolin-7-glucoside were isolated for the first time from S. plebeia. (c) 2008 Elsevier B.V. All rights reserved.